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We study the cilia driven flow of the cerebrospinal fluid in the ventral third ventricle of the mammalian brain.
The beating cilia create a complex flow network and we use fluorescent bead tracking, differential interference contrast imaging of beating cilia,
and immunostaining including subsequent quantitative image analysis to describe and consequently understand the flow network.
[1] R. Faubel, C. Westendorf, E. Bodenschatz and G. Eichele, Science, 353, 176-178 (2016). [2] G. Eichele, E. Bodenschatz, Z. Ditte, A-K. Günther, S. Kapoor, Y. Wang and C. Westendorf, Phil. Trans. of the Royal Society B, 375(1792), (2019). |
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Beating cilia bundles. Cilia bundles line the ventricular wall and partially drive the flow of the cerebrospinal fluid.
Recorded with DIC microscopy at 100 Hz.
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Full flowmap of the ventral third ventricle ependyma. Each line represents the track of one fluorescent bead driven across the ependymal surface by the beating cilia.
Color indicates the direction of the respective track segment and the arrows mark the flow direction as a guide to the eye.
ac - anterior commissure, tz - tanyzyte region, S - separatrix (opposing flows).
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