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Single amoeboid cells need to navigate in a complex environment by receiving and integrating information and translating those information into directed migration.
This is in particular necessary for Dictyostelium discoideum and other cellular slime molds, in order to form a multicellular organism out of single scattered ameba. We (together with Carsten Beta, Uni Potsdam, and other co-workers of the LFPB department) investigate the dynamics of the eukaryotic cytoskeleton by applying time varying stimuli to individual Dictyostelium cells. The different pulse and periodic input responses yield information on the probed system and allowed us to describe the cytoskeletal response in the context of non-linear dynamics. |
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[1] C. Westendorf, J. Negrete Jr., A. Bae, R. Sandmann, E. Bodenschatz and C. Beta,
Proc. Natl. Acad. Sci. USA, (2013). [2] J. Negrete Jr., A. Pumir, H.F. Hsu, C. Westendorf, M. Tarantola, C. Beta and E. Bodenschatz, Phys. Rev. Lett., (2016). |
[3] H.F. Hsu, E. Bodenschatz, C. Westendorf, A. Gholami, A. Pumir, M. Tarantola and C. Beta,
Phys. Rev. Lett., (2017). [4] J. Negrete Jr., A. Pumir, C. Westendorf, M. Tarantola, E. Bodenschatz and C. Beta, Phys. Rev. Research, (2020). |
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Exemplary experiment. The D. discoideum cell is exposed to regular pulses of chemoattractant through microfluidic flow photolysis (Stimulus = red).
The actin cytoskeleton is fluorescently labeled and the response function (blue) is given by the average fluorescence intensity in the cell interior (excluding the cortex).
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